Segments

The group, Hi-5, performs songs as a group as well as presenting individual segments. All segments are integrated with music as a tool to highlight the key concepts of each episode.

The Shapes in Space segment focuses on visual and spatial awareness, with the presenter exploring shapes, colour and everyday materials such as boxes and play-dough. Musicality is explored through Making Music, with an emphasis on pitch, rhythm, beat, melody, and using a variety of real and pretend instruments. The presenter of Body Move encourages children to participate in movement and dance, developing physical coordination and motor development. Linguistics and aural skills are at the centre of the Word Play segment, featuring a puppet named Chatterbox who assists in the exploration of language through stories and rhymes. Puzzles and Patterns has a focus on logical thinking and mathematics, with a puppet named Jup Jup used as a tool for the presenter to complete puzzles or solve problems.

The final segment in which the cast comes together is entitled Sharing Stories, where a story is told that explores interpersonal relationships and emotions. The episodes are book-ended with a Song of the Week; a pop-style feature song which corresponds with the weekly theme and sets an educational topic for the week's episodes.

The 2017 revived series featured new puppet characters, The Jupsters, who were introduced as the family of previously established character Jup Jup. The revival also retained a segment introduced in Hi-5 House, entitled The Chatterbox. This segment focuses on the discovery of language through simple words and phrases, and features the puppet Chatterbox, who teaches a toy robot named Tinka how to speak.

Body Move
Hosted by:
 * Charli Robinson (Series 1-10)
 * Casey Burgess (Hi-5 Playtime! Concert)
 * Lauren Brant (Series 11-13)
 * Mary Lascaris (Hi-5 House)
 * Bailey Spalding (Series 17)

Making Music
Hosted by:
 * Tim Harding (Series 1-9)
 * Stevie Nicholson (Series 10)
 * Tim Maddren (Series 11-13)
 * Ainsley Melham (Hi-5 House)
 * Joe Kalou (Series 17)

Puzzles & Patterns
Hosted by:
 * Kathleen de Leon Jones (Series 1-8)
 * Sun Pezzimenti (Series 9-10)
 * Fely Irvine (Series 11-13)
 * Dayen Zheng (Hi-5 House)
 * Shay Clifford (Series 17)

Shapes In Space
Hosted by:
 * Nathan Foley (Series 1-10)
 * Stevie Nicholson (Series 11-13, Hi-5 House)
 * Lachie Dearing (Series 17)

Word Play
Hosted by:
 * Kellie Crawford (Series 1-10)
 * Casey Burgess (Series 11-13)
 * Lauren Brant (Hi-5 House Series 1)
 * Tanika Anderson (Hi-5 House Series 2-3)
 * Courtney Clarke (Series 17)

Fit Bit Tips
Hosted by:
 * Nathan Foley (Series 10)
 * Stevie Nicholson (Series 10-12)
 * Tim Maddren (Series 11-12)

The Chatterbox
Hosted by:
 * Chatterbox (Hi-5 House and Series 17)

The Jupsters
Hosted by:
 * Chatterbox, Jup Jup, Jard Jard, Puj Puj, Jaan Jaan, Jor Jor, Jed Jed & Jru Jru (Series 17)